The results indicated a significant influence of type of explant and copper on callus induction in pineaple cultivars. BAP and / or 2,4-D have been added to base medium for calli maturation and BAP and GA3 for plant regeneration. Murashige and Skoog base medium supplemented with vitamins B5 and different in hormonal combinations: Auxins / Cytokins. Thus, calli were induced from crown leaf and plantlets leaf of Smooth Cayenne and Sugar Loaf cultivars. The aim of this study was to evaluate the responses of two cultivars of pineapple regenerated through somatic embryogenesis. However, somatic embryogenesis (SE) have been revealed the most rapid and controllable method for Pineapple propagation than conventional sucker production methods. Pineapple production is mostly constrained by unavailability of high-performance suckers. The Freeze-thawing method can be used to solubilize FPOX inclusion bodies at lower concentrations of urea. In conclusion, FPOX inclusion bodies are heterogenous in size and can be decreased using signal peptide. For rFPOX activity, TMB was used as coloring agent for the first time in HbA1c enzymatic method as peroxidase substrate. The rFPOX was successfully secreted in the periplasmic space using PelB signal peptide that shown the amount of periplasmic protein in shuffle was higher than of BL21. By using the stabilizers on solubility of rFPOX we found that the effect of sorbitol was higher than arginine. By freeze-thawing method, the inclusion bodies was soluble in urea 8 M, but was not soluble in urea 0.5 M. The inclusion bodies of FPOX were only soluble in urea 8 M, while was not soluble in any of used concentrations of DMSO solvent. Recombinant FPOX was mainly expressed as inclusion bodies.
coli and secretion of the enzyme into periplasmic space using PelB signal peptide were investigated. In this study, the methods for decreasing the formation of Fructosyl Peptide Oxidase (FPOX) inclusion bodies in E. HbA1c enzymatic method has been considered as a popular method for determination of blood glucose.
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